Accurate protein quantification is essential in life science research, from enzyme activity studies and protein–protein interaction assays to sample preparation for advanced downstream analyses. The BCA Protein Estimation Kit provides a dependable solution, delivering precise, reproducible results across a wide range of biological samples, including cell lysates, serum, plasma, and urine.
Using the well-established bicinchoninic acid (BCA) chemistry, the assay produces a stable purple color when protein–copper complexes are formed under alkaline conditions. The intensity of this color, measurable at 562 nm, directly correlates with protein concentration, ensuring consistent and quantitative assessment.
This kit is supplied with following components:
| Reagent |
Catalog No. |
Storage |
| A |
PX-RGA-101 |
Room Temperature |
| B |
PX-RGB-101 |
| Protein Standard |
PX-PS-101 |
2-8℃ |
| SPECIFICATION |
| Specification |
Description |
| Method |
BCA (Bicinchoninic Acid) Protein Assay |
| Reference Protein |
BSA (Bovine Serum Albumin), 2 mg/mL |
| Intended Use |
Solution-based protein detection, absorbance measurement |
| Instruments |
Spectrophotometer, Microplate Reader |
| Components |
10 × 1 mL glass ampoules |
| Category |
Protein Quantitation Assay |
| Pack Size |
1 Kit (250 ml) and (500ml) |
| Target Range |
General protein quantification (not target-specific) |
| Throughput |
~250 cuvette assays and ~500 cuvette assays |
| Readout |
Colorimetric (absorbance at 562 nm) |
| Packaging Unit |
Each |
Key Advantages
Broad Compatibility – Works effectively in the presence of ionic and non-ionic detergents, making it ideal for lysates and extraction buffers.
High Accuracy – Less affected by protein-to-protein variation than dye-binding assays such as Bradford.
Convenient & Efficient – Simple protocol with minimal hands-on time; results in under 30 minutes.
Flexible Format – Sufficient for ~2,500 microplate reactions or 500 cuvette assays.
