Proteogenixx’s RIPA (Radioimmunoprecipitation Assay) Buffer is a high-quality, ready-to-use reagent designed for effective cell lysis and protein extraction from mammalian cells.It offers excellent performance at an affordable cost, eliminating the need for additional preparation.
RIPA Buffer efficiently disrupts cell membranes while preserving protein integrity, making it ideal for applications such as Western blotting, immunoprecipitation, and protein quantification assays. Its optimized formulation—containing a balanced blend of buffers, salts, and detergents—ensures efficient solubilization of both cytoplasmic and nuclear proteins.
For best results, it is recommended to keep all samples and buffers ice-cold during use to minimize enzymatic activity and protect protein structure, ensuring high-quality extracts suitable for accurate downstream analyses.
Storage: Store at 2–8 °C; keep buffer ice-cold during use to preserve protein integrity.
| SPECIFICATION |
| Specification |
Description |
| Assay / Application |
Cell lysis, protein extraction, Western blotting, Immunoprecipitation, BCA Assay |
| Concentration |
Ready-to-use formulation |
| For Use With (Application) |
Protein solubilization and extraction from mammalian cells |
| For Use With (Equipment) |
General laboratory equipment, spectrophotometer (for downstream assays) |
| Kit / Buffer Contents |
500 mL solution (organic buffers, salts, detergents, stabilizers) |
| Product Type |
Protein Extraction |
| Quantity |
100 mL, 250mL &500 mL |
| Specificity |
Not target-specific (broad-spectrum protein extraction) |
| Adequate For |
Multiple protein extraction reactions depending on protocol |
| Detection Method |
Compatible with colorimetric protein quantification (e.g., BCA assay) |
| Unit Size |
Each |
Key Features & Advantages
Ready-to-Use – Supplied as a pre-formulated solution; no preparation required.
High Efficiency – Effective cell lysis and solubilization of diverse protein classes.
Applications – Western blotting, immunoprecipitation, BCA protein assays, and more.
Protein Integrity – Maintains protein stability during extraction.
Convenience – Saves time, reduces costs, and ensures consistent results.
