Coomassie Brilliant Blue (CBB) staining is one of the most widely adopted techniques for protein detection in polyacrylamide gels, particularly SDS-PAGE. Known for its simplicity, efficiency, and affordability, CBB staining remains a staple method in molecular biology and biochemistry laboratories.
The dye interacts primarily with basic amino acids such as arginine, lysine, and histidine, forming stable dye–protein complexes that appear as distinct blue protein bands against a clear gel background. The method allows reliable detection of proteins at levels as low as 30–100 ng, depending on the formulation.
The CBB Ultra formulation enhances this classical method by offering ultra-sensitive detection with a simplified workflow. It eliminates the need for gel fixing and destaining steps—protein bands appear within 5–10 minutes after rinsing with water, providing rapid and clear visualization with minimal background. Importantly, the stain is also fully compatible with mass spectrometry-based protein identification, making it suitable for proteomic workflows.
This kit is supplied with following components:
| Component |
Catalog No. |
Storage |
| CBB Staining Solution |
PX-CCBB-301 |
Room Temperature |
| SPECIFICATION |
| Specification |
Description |
| Assay |
Protein staining in SDS-PAGE gels |
| Concentration |
Ready-to-use solution |
| For Use With (Application) |
Visualization of proteins in polyacrylamide gels |
| For Use With (Equipment) |
Gel electrophoresis apparatus, imaging systems |
| Kit / Buffer Contents |
CBB Colloidal Staining Solution (ultra-sensitive Coomassie-based stain) |
| Product Type |
Protein Visualization Reagent |
| Quantity |
500 mL& 1L |
| Sufficient For |
Multiple SDS-PAGE gel staining runs |
| Detection Method |
Colorimetric (blue protein bands on gel background) |
| Unit Size |
Each |
| Storage |
Store at 2–8 °C |
Key Features & Advantages
Detection Sensitivity – 30–100 ng of protein
Fast Protocol – Clear bands in 5–10 minutes.
Workflow – No fixing or destaining required.
Compatibility – Mass spectrometry–friendly
Applications – Routine SDS-PAGE protein detection and proteomics
Cost-Effective – Simple and economical solution for everyday protein analysis
